Porto's recent run of offensive form in their last three games, scoring 12 goals and conceding none, will try and reverse that chance to give their side a chance.
After earning a 2-0 win in the first leg on the road, Juve won the second leg against the 10-man Portuguese side 1-0 to go through 3-0 on aggregate.
As expected, Leonardo Bonucci started for Juventus after missing the first leg following a disagreement with head coach Allegri.
Miralem Pjanic was also left on the bench as Allegri selected an attacking team which included Dybala, Gonzalo Higuain and Mario Mandzukic. Porto was in defensive mode after a 27th minute banishment by Alex Telles.
"For us, it's a different situation", Nuno said when asked if Barcelona's second-leg performance had inspired his side.More news: Alshon Jeffery headlines new-look receiver group for Eagles, Carson Wentz
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The Croatian striker, though, could not direct his downward header past veteran Porto keeper Iker Casillas, who overtook Paolo Maldini at the top of the all-time Uefa club appearances list with 175 games.
They got the goal they deserved in the 42nd minute when Dybala expertly dispatched a penalty into the bottom right corner, after Maxi Pereira was shown a straight red card for a deliberate handball as he stopped Higuain's attempt from going over the line.
Both managers made changes for the second half as Porto replaced forward Andre Silva with defender Willy Boly to rejig the backline, while Juventus took off Cuadrado as Marko Pjaca came on.
Porto then immediately broke and should have been on level terms when Francisco Soares went one-on-one with Gianluigi Buffon, but his shot went wide of the far post.